Last edited by Samusho
Wednesday, July 22, 2020 | History

6 edition of Homing Endonucleases and Inteins (Nucleic Acids and Molecular Biology) (Nucleic Acids and Molecular Biology) found in the catalog.

Homing Endonucleases and Inteins (Nucleic Acids and Molecular Biology) (Nucleic Acids and Molecular Biology)

  • 350 Want to read
  • 15 Currently reading

Published by Springer .
Written in English

    Subjects:
  • Genetics (non-medical),
  • Life Sciences - Genetics & Genomics,
  • Engineering - Chemical & Biochemical,
  • Science,
  • Science/Mathematics,
  • Life Sciences - Biochemistry,
  • Mobile Intein,
  • Mobile Intron,
  • Protein Engineering,
  • Protein Splicing,
  • Science / Biochemistry,
  • Life Sciences - Biology - Molecular Biology,
  • Endonucleases,
  • Inteins

  • Edition Notes

    ContributionsMarlene Belfort (Editor), Barry L. Stoddard (Editor), David W. Wood (Editor), Victoria Derbyshire (Editor)
    The Physical Object
    FormatHardcover
    Number of Pages377
    ID Numbers
    Open LibraryOL9055387M
    ISBN 103540251065
    ISBN 109783540251064

    Books New Releases Best Sellers Arabic Books Children's Books Business & Finance Self-Help Literature & Fiction Cookbooks Biographies & Memoirs Homing Endonucleases and Inteins. by Marlene Belfort, Barry L. Stoddard, et al. | 22 September Paperback. Homing Endonucleases. Many inteins include two parts: a portion that splices the intein out of the overall protein chain, and a portion that acts as a DNA-cutting enzyme. This enzyme is often termed a homing endonuclease because it homes in on DNA that doesn't encode an intein. Homing endonucleases act as selfish genetic elements, with an.

    Self splicing introns and inteins that rely on a homing endonuclease for propagation are parasitic genetic elements. Their life-cycle and evolutionary fate has been described through the homing cycle. According to this model the homing endonuclease. own reading frames. The genes that encode homing endonucleases are often embedded within self-splicing elements such as group I introns, group II introns and inteins. This combination of molecular functions is mutually advantageous: the endonuclease activity allows surrounding introns and inteins to act as invasive DNA elements, while the splicing.

    Introduction. Homing endonucleases (HEs) are DNA-cutting enzymes that are encoded by homing endonuclease genes (HEGs). HEGs can be free-standing genes but they have been identified to be encoded within archaeal introns, and they are frequently embedded within self-splicing elements such as group I, group II introns, and inteins (Gimble ; Gogarten et al. ; Belfort et al. ; Barzel et. Homing endonucleases are encoded by open reading frames that are embedded within group I, group II and archael introns, as well as inteins (intervening sequences that are spliced and excised post-translationally). These enzymes initiate transfer of those elements (and themselves) by generating stran .


Share this book
You might also like
divine liturgy, the Sunday Epistles & Gospels.

divine liturgy, the Sunday Epistles & Gospels.

Official report of evidence taken during the session of 1921 respecting future fuel supply of Canada.

Official report of evidence taken during the session of 1921 respecting future fuel supply of Canada.

The satires of Decimus Junius Juvenalis

The satires of Decimus Junius Juvenalis

field guide to the loess deposits and channeled scablands of the Palouse area, eastern Washington

field guide to the loess deposits and channeled scablands of the Palouse area, eastern Washington

Prevention of Terrorism Bill

Prevention of Terrorism Bill

Contributions to the swinging festival in Western Central India by Rita Wiesinger and Josef Haekel.

Contributions to the swinging festival in Western Central India by Rita Wiesinger and Josef Haekel.

Metafisica dos tubos

Metafisica dos tubos

1973 accidents of motor carriers of property.

1973 accidents of motor carriers of property.

Constitution, by-laws and rules of order

Constitution, by-laws and rules of order

The ruins

The ruins

protection of corn, November 1984 - April 1988

protection of corn, November 1984 - April 1988

Exposition of Psalm CXIX

Exposition of Psalm CXIX

GCSE and GCE(A/AS)

GCSE and GCE(A/AS)

Banking question in the United States

Banking question in the United States

St. Martin-in-the-Fields.

St. Martin-in-the-Fields.

Cyprian

Cyprian

Homing Endonucleases and Inteins (Nucleic Acids and Molecular Biology) (Nucleic Acids and Molecular Biology) Download PDF EPUB FB2

This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins.

This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins.

These two unique protein superfamilies, which are tied together through their frequent. Although HO is related to inteins, and more distantly related to other homing endonucleases in the LAGLIDADG superfamily (Chevalier and Stoddard, ), it is an independently expressed standalone.

Get this from a library. Homing Endonucleases and Inteins. [Marlene Belfort; Barry L Stoddard; David W Wood] -- This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and.

The homing endonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing catalyze the hydrolysis of genomic DNA within the cells that synthesize them, but do so at very few, or even singular, locations.

Repair of the hydrolyzed DNA by the host cell frequently results in the gene encoding the. Homing Endonucleases. The homing endonuclease is another strange and relatively rare type of mobile DNA that is not a transposable element.

Homing endonucleases are found in three situations: free standing or encoded within either inteins (see Section ) or introns. They mobilize themselves, plus, in the latter two situations, the. Homing endonucleases are double stranded DNases that have large, asymmetric recognition sites ( base pairs) and coding sequences that are usually embedded in either introns or inteins (1).

Introns are spliced out of precursor RNAs, while inteins are spliced out of precursor proteins (2,3). Furthermore, Homing Endonucleases and Inteins book distribution of homing endonucleases among intervening sequences suggests the origin of homing endonucleases and intervening sequences to also be independent (reviewed in 13).

For example, homologous members of both the LAGLIDADG and HNH families are found in unrelated introns and by:   Self splicing introns and inteins that rely on a homing endonuclease for propagation are parasitic genetic elements.

Their life-cycle and evolutionary fate has been described through the homing cycle. According to this model the homing endonuclease is selected for function only during the spreading phase of the parasite.

This phase ends when the parasitic element is fixed in the population. This book provides a comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins.

Rating: (not yet rated) 0 with reviews - Be the first. Homing endonucleases are strong drivers of genetic exchange and horizontal transfer of both their own genes and their local genetic environment.

The mechanisms that govern the function and evolution of these genetic oddities have been well documented over the past few decades at the genetic, biochemical, and structural levels. Homing endonucleases are encoded by open reading frames that are embedded within group I, group II and archael introns, as well as inteins (intervening sequences that are spliced and excised post-translationally).

These enzymes initiate transfer of those elements. Class 5: Homing Endonucleases, Introns and Inteins. Homing Endonucleases. Homing Endonucleases are selfish DNAs that spread to homologous sites in other phages; HNH Structure; T4; Homing Endonucleases in T4; Introns.

Introns; Introns are Self-Splicing Elements; HNH gene in Group I Self-Splicing Introns in T4; Secondary Structure at RBS in HNH Genes. To the download Homing Endonucleases and Inteins we may as be what is to the North hands, in the beggars, in the laws of the fellow-citizens, and in the parties, it has relative to express that the ships of the case, of the Grafio or general commerce, and the Centenarius, annexed the public; that the taxes, the Rathimburgers, and the states /5.

Goodrich-Blair H, Shub DA () Beyond homing: competition between intron endonucleases confers a selective advantage on flanking genetic markers. Cell – PubMed Google Scholar Gorbalenya AE () Self-splicing group I and group II introns encode homologous (putative) DNA endonucleases of.

Meganucleases are endodeoxyribonucleases characterized by a large recognition site (double-stranded DNA sequences of 12 to 40 base pairs); as a result this site generally occurs only once in any given example, the base pair sequence recognized by the I-SceI meganuclease would on average require a genome twenty times the size of the human genome to be found once by chance.

For more information, click on the following subjects: The Intein Registry: A. Inteins Listed in Order of Phylogenetic Domain of Life, Genus and Species B. Inteins Alleles = Inteins Grouped by Extein Insertion Site C. Selected Intein Properties Please note: Finding DOD homing endonuclease motifs is not sufficient evidence that the gene contains an intein since DOD homing endonucleases are also.

Books & Chapters. Edited Volume. Belfort, M., Derbyshire, V., Stoddard, B.L. & Wood, D.W. (Eds.), Homing Endonucleases and : Nucleic Acids and Molecular. Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins Hideo Iwaï1, Kornelia M.

Mikula1, Jesper S. Oeemig1,4, Dongwen Zhou2,5, Mi Li2,3 and Alexander Wlodawer2 1 - Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O.

Helsinki. Belfort M, Bonocora RP. Homing endonucleases: from genetic anomalies to programmable genomic clippers. Methods Mol Biol. ; Bonocora RP, Belfort M. Mapping homing endonuclease cleavage sites using in vitro generated protein. Homing endonucleases are encoded by open reading frames that are embedded within group I, group II and archael introns, as well as inteins (intervening sequences that are spliced and excised post-translationally).

These enzymes initiate transfer of those elements (and themselves) by generating strand breaks in cognate alleles that lack the.The internal fragments of precursor proteins (INternal proTEINS) that are autocatalytically removed by PROTEIN SPLICING.

The flanking fragments | Explore the latest full-text research PDFs.We have used the homing intron to illustrate the movement of homing endonucleases (Fig. ). The movement of homing endonucleases that are located inside inteins or are free standing is very similar. The homing endonuclease cleaves the recognition sequence at the target site and generates short overhanging ends.